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The amount of xGen UDI-UMI Adapters you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend checking your specific protocol for the optimal amount of adapter to use.
For the xGen DNA Library Prep Kits, use 5 µL of xGen UDI-UMI Adapters (diluted according to the table below) for the ligation reaction.
Note: If diluting the xGen Stubby Adapter is required, we recommend using Nuclease-Free Duplex Buffer.
| DNA input (ng) | Adapter dilution | Stock concentration (µM) |
|---|---|---|
| ≥25 | No dilution | 15 |
| 10 | 10-fold (1:10) | 1.5 |
| 1 | 20-fold (1:20) | 0.75 |
Reach out to our Scientific Applications Support team if you are unsure.